Regulatory

Part:BBa_K318514:Design

Designed by: Sarah R. Sandock   Group: iGEM10_Wisconsin-Madison   (2010-09-26)

acrRA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 471
    Illegal XhoI site found at 785
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The acrRA operon is found in Salmonella enterica strain LT2. This operon contains the RamA binding sequence, the acrR gene and part of the acrA gene.

Source

The Salmonella enterica strain LT2 chromosomal DNA came from Diana Downs' Lab at the University of Wisconsin-Madison.

References

Nikaido E., A. Yamaguchi, and K. Nishino. 2008. AcrAB Multidrug Efflux Pump Regulation in Salmonella enterica serovar Typhimurium by RamA in Response to Environmental Signals. J. Biol. Chem. 283:24245-24253.